Micro propagation

 MICROPROPAGATION:-

                                    It has been demonstrated that a variety of plant can be conveniently propagated through the techniques of cell, tissue organ culture. This is popularly described as micro propagation.

Technique of Micro propagation:-

                          In most of cases, clone propagation is achieved by placing sterilized short tips or axillary buds on to a culture medium that is sufficient to induce formation of multiple buds. 

Following stage are involved in the method of micro propagation 

  1. Stage I involves establishment of tissue in vitro.

  2. Stage II involve multiplication of shoots.

  3. Stage III concerned root formation and conditioning of propagules prior to transfer to the greenhouse. This stage requires high intensity and alteration of media for promotion of  root formation. 

   4. Stage IV involves growth in pots followed by field trials.

Advantages of Micro propagation:-   

• Rapid multiplication of superior clones and maintenance of uniformity.                                                                                                
• Multiplication of disease free plant.                                                         
• Multiplication of sexually derived sterile hybrids.                                                      
• A wide range of plants have now been regenerated through technique of tissue culture. This technique is very useful in the    commercial production of some plants like banana, potato, orchids and Chrysanthemum etc.