A good host have the following features:-
- Should be easy transform,
- Should support the replication of recombinant DNA,
- Should be free from elements that interfere with replication of recombinant DNA,
- Lack active restriction enzyme, e.g. E.coli K12 sub strain HB 101,
- Should not have Methylases since these enzyme would methylate the replicated recombinant DNA which, as a result, would become resistant to useful restriction enzymes,
- Should be deficient in normal recombination function so that the DNA insert is not altered by recombination events. E.coli support several type of vectors, some natural, constructed, which can be grouped as follows:- 1. Plasmid 2. Bacteriophages (both natural) 3. Cosmids, 4. Phasmids 5. Shuttle vectors, 6. Artificial chromosomes 7. Phagemids,