Polymerase Chain Reaction (PCR) Quiz – Part 4: Numerical & Analytical Questions

Attempt all 10 questions. Click Submit to check your score and correct answers.

31. Which factor MOST affects PCR specificity?

a) dNTP concentration
b) Annealing temperature
c) Polymerase concentration
d) Buffer pH

32. Hot-start PCR prevents:

a) Primer degradation
b) Nonspecific amplification
c) Template denaturation
d) Enzyme inactivation

33. Which additive is often used to improve PCR amplification of GC-rich templates?

a) Glycerol
b) DMSO
c) NaCl
d) EDTA

34. Which enzyme is commonly used for high-fidelity PCR?

a) Taq polymerase
b) Pfu polymerase
c) Reverse transcriptase
d) DNA ligase

35. Which PCR type is BEST suited for identifying rare mutations in a sample?

a) Nested PCR
b) qPCR
c) Digital PCR
d) Multiplex PCR

36. Which PCR technique allows amplification without thermal cycling?

a) LAMP (Loop-mediated isothermal amplification)
b) Touchdown PCR
c) Nested PCR
d) Hot-start PCR

37. Which application commonly uses reverse transcriptase PCR?

a) Gene expression studies
b) DNA fingerprinting
c) Protein purification
d) Plasmid cloning

38. Which PCR method allows simultaneous detection of multiple pathogens?

a) Nested PCR
b) Multiplex PCR
c) Hot-start PCR
d) Digital PCR

39. Which step in PCR is MOST heat-sensitive?

a) Denaturation
b) Annealing
c) Extension
d) Template storage

40. Which variant of PCR is widely used in next-generation sequencing (NGS) library preparation?

a) qPCR
b) Bridge PCR
c) Nested PCR
d) Allele-specific PCR

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