Polymerase Chain Reaction (PCR) Quiz – Part 2: Techniques & Applications

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11. Which PCR variant is used for amplifying RNA templates?

a) Nested PCR
b) RT-PCR
c) qPCR
d) Hot-start PCR

12. Which of the following applications uses PCR?

a) Forensic DNA profiling
b) Gene cloning
c) Pathogen detection
d) All of the above

13. Nested PCR improves specificity by:

a) Using two sets of primers in successive runs
b) Using degenerate primers
c) Increasing Mg²⁺ concentration
d) Reducing annealing temperature

14. Real-time PCR monitors amplification by measuring:

a) Radioactive isotopes
b) Absorbance
c) Fluorescence
d) Enzyme activity

15. Which dye binds to double-stranded DNA in qPCR?

a) SYBR Green
b) Coomassie Blue
c) Safranin
d) Crystal violet

16. Which PCR variant is best for detecting a single nucleotide polymorphism (SNP)?

a) Allele-specific PCR
b) RT-PCR
c) Nested PCR
d) Touchdown PCR

17. Which PCR modification reduces nonspecific amplification by gradually lowering the annealing temperature?

a) Hot-start PCR
b) Touchdown PCR
c) Digital PCR
d) Multiplex PCR

18. Multiplex PCR allows:

a) Amplification of multiple targets in one reaction
b) Amplification of a single target with multiple enzymes
c) Extension without primers
d) DNA sequencing

19. Which of the following is NOT an application of PCR?

a) Gene expression analysis
b) DNA fingerprinting
c) Protein purification
d) Detection of pathogens

20. Digital PCR is mainly used for:

a) High-throughput sequencing
b) Absolute quantification of DNA
c) Gene cloning
d) Protein expression

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