Saturday, 22 May 2021

Functions of carbohydrates

Carbohydrates are one of the most abundant and diverse classes of biomolecules in living organisms. Chemically, they are polyhydroxy aldehydes or ketones and their derivatives. Biologically, carbohydrates play essential roles in energy metabolism, structural organization, cellular communication, and regulation of physiological processes. Due to their versatility and abundance, carbohydrates are indispensable for the survival of all forms of life, from microorganisms to higher eukaryotes.

1. Carbohydrates as a Primary Source of Energy

The most fundamental function of carbohydrates is their role as the primary source of metabolic energy. Upon complete oxidation, carbohydrates yield approximately 4 kcal g⁻¹, making them an efficient and readily available energy source. Glucose is the central carbohydrate in energy metabolism and serves as a universal fuel for cells.

In aerobic organisms, glucose undergoes glycolysis, producing pyruvate, ATP, and reducing equivalents (NADH). Pyruvate is further oxidized through the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, leading to maximum ATP generation. Certain tissues such as the brain, red blood cells, and renal medulla depend almost exclusively on glucose for energy. In plants, carbohydrates synthesized during photosynthesis act as the primary energy source for all heterotrophic organisms in the biosphere.

2. Role as Energy Storage Molecules

Carbohydrates also function as storage forms of energy, ensuring a continuous supply of fuel during periods of fasting or increased energy demand. In animals, excess glucose is stored as glycogen, primarily in the liver and skeletal muscles. Liver glycogen maintains blood glucose levels, while muscle glycogen provides energy for muscle contraction.

In plants, carbohydrates are stored mainly as starch, composed of amylose and amylopectin. Starch serves as a long-term energy reserve in seeds, tubers, and roots. Storage polysaccharides are osmotically inert and compact, allowing efficient energy storage without disrupting cellular homeostasis.

3. Structural Role of Carbohydrates

One of the most important functions of carbohydrates is their role as structural components of cells and tissues. Structural polysaccharides provide mechanical strength, rigidity, and protection.

  •    Cellulose, a linear polymer of ฮฒ-D-glucose, is the major structural component of plant cell walls and contributes to cell shape and resistance against mechanical stress.
  •     Chitin, composed of N-acetylglucosamine units, forms the exoskeleton of arthropods and the cell walls of fungi.
  •   In bacteria, carbohydrates are integral components of the cell wall, such as peptidoglycan, which maintains cell shape and prevents osmotic lysis.

Thus, carbohydrates play a vital role in maintaining the integrity and architecture of biological systems.

4. Precursors of Other Biomolecules

Carbohydrates serve as metabolic precursors for the synthesis of numerous biologically important molecules. Intermediates of carbohydrate metabolism are diverted into various biosynthetic pathways.

  •   Fatty acids and lipids are synthesized from acetyl-CoA derived from carbohydrate catabolism.
  •      Amino acids such as alanine, glycine, and serine are formed from glycolytic intermediates.
  •     Ribose-5-phosphate, generated through the pentose phosphate pathway, is essential for the synthesis of nucleotides, nucleic acids, and coenzymes (NAD⁺, FAD, ATP).

Therefore, carbohydrates act as central hubs connecting catabolic and anabolic pathways.

5. Role in Cell Membrane Structure and Function

Carbohydrates are key components of the cell membrane, where they are present as glycoproteins and glycolipids. These carbohydrate-containing molecules are primarily located on the extracellular surface of the plasma membrane, forming the glycocalyx.

The glycocalyx plays critical roles in:

  •     Cell–cell recognition and adhesion
  •     Signal transduction
  •     Immune response
  •     ·Fertilization and development

For example, blood group antigens (ABO system) are determined by specific carbohydrate moieties present on red blood cell membranes. Similarly, pathogen recognition by host cells often involves carbohydrate–protein interactions.

6. Role in Cell Communication and Signaling

Carbohydrates participate in cellular communication by serving as recognition markers and ligands for receptors. Lectins, a class of carbohydrate-binding proteins, specifically recognize carbohydrate residues and mediate biological processes such as immune responses, inflammation, and cell migration.

Glycosylation of proteins influences their folding, stability, targeting, and function. Improper glycosylation is associated with several diseases, including congenital disorders of glycosylation and cancer.

7. Protective and Lubricating Functions

Certain carbohydrates and their derivatives have protective and lubricating roles. Mucopolysaccharides or glycosaminoglycans (e.g., hyaluronic acid, chondroitin sulfate) are major components of connective tissues, synovial fluid, and cartilage.

Hyaluronic acid acts as a lubricant in joints, while chondroitin sulfate provides tensile strength to cartilage. These molecules also play roles in wound healing and tissue repair.

8. Role in Osmoregulation and Detoxification

Carbohydrates help in maintaining osmotic balance within cells. Some sugars and sugar alcohols act as compatible solutes in microorganisms and plants, protecting cells from osmotic stress.

Additionally, carbohydrates participate in detoxification processes. Glucuronic acid conjugates with toxic substances, drugs, and bilirubin in the liver, facilitating their excretion from the body.

Conclusion

In conclusion, carbohydrates are multifunctional biomolecules essential for life. They serve as primary sources and storage forms of energy, provide structural support, act as precursors for biosynthesis, and play crucial roles in cell membrane architecture, communication, and protection. Their involvement in metabolic regulation, development, immunity, and homeostasis highlights their central importance in biological systems. A thorough understanding of carbohydrate functions is fundamental for advanced studies in biochemistry, molecular biology, and biotechnology, particularly for competitive examinations such as CSIR-NET and GATE.

 


CSIR-NET MCQs: Carbohydrates

CSIR-NET MCQs: Functions of Carbohydrates

1. Major energy-yielding carbohydrate in human metabolism is:




Explanation: Glucose is the universal fuel molecule and primary substrate for glycolysis.

2. Structural polysaccharide in plant cell wall is:




Explanation: Cellulose is a ฮฒ-1,4-linked glucose polymer providing rigidity to plant cell walls.

3. Glycogen is mainly stored in:




Explanation: Liver glycogen maintains blood glucose, while muscle glycogen supplies local energy.

4. Pentose phosphate pathway mainly provides:




Explanation: PPP supplies NADPH for reductive biosynthesis and ribose for nucleotide synthesis.

5. Glycoproteins are mainly involved in:




Explanation: Carbohydrate moieties on glycoproteins mediate cell–cell recognition and signaling.

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