Steps in GENE CLONING

Steps in Gene Cloning:-

The entire procedure of gene cloning or recombinant DNA technology may be classified into the following five steps the convenience in description and on the basis of the chief activity performed.

1. Production and isolation of the DNA fragments to cloned.                     
2. Insertion of the isolated gene in a suitable vector to obtain recombinant DNA.                                                                                  
3. Introduction of the recombinant DNA into a suitable organism/cell usually we used E. coli  called host(transformation).                                                                                 
4. Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment.                                   
5. Multiplication/expression of the introduced gene in the host.                    
6. Where needed, transfer and expression of the gene in the another organism.