Analytical/Preparative Centrifugation:-
The 2 most common types of centrifugation are analytical and preparative; the distinction is between the 2 is based on the purpose of centrifugation. Analytical centrifugation involves measuring the physical properties of the sedimenting particles, such as sedimentation coefficient or molecular weight. Optimal methods are used in analytical ultracentrifugation. Molecules are observed by optical system during centrifugation, to allow observation of macromolecules in solution as they move in the gravitational field. The samples are centrifuged in cells with windows that lie parallel to the plane of rotation of the rotor head. As the rotor turns, the images of the cell (proteins) are projected by an optical system onto film or a computer. The concentration of the solution at various points in the cell is determined by absorption of a light of the appropriate wavelength. This can be accomplished either by measuring the degree of blackening of a photographic film or by the deflection of the recorder of the scanning system and fed into a computer. The other type of centrifugation is called preparative and the objective is to isolate specific particles that can be reused. There are many type of preparative centrifugation such as rate zonal, differential, and isopycnic centrifugation.
.png)
